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GIPSA Verifies Performance of Test Kits to Detect Aflatoxin and Deoxynivalenol (DON)

The USDA Grain Inspection, Packers and Stockyards Administration has verified a number of qualitative and quantitative test kits

August 10, 2006


The USDA Grain Inspection, Packers and Stockyards Administration has verified a number of qualitative and quantitative test kits

The USDA Grain Inspection, Packers and Stockyards Administration has verified that the following qualitative test kits for detecting the presence of mycotoxins at the specified levels:

Charm Sciences, Inc. ROSA® DON P/N (Product #LF-DONPN) - Deoxynivalenol in wheat and barley at 1 ppm
Charm Sciences Inc. ROSA® DON P/N (Product #LF-DONPN) - Deoxynivalenol in wheat at 0.5 ppm
Charm Sciences Inc. ROSA® Aflatoxin P/N (Product #LF-AFPN) - Aflatoxin in corn at 10 ppb
Diachemix, LLC DON FPA (Product #61530) - Deoxynivalenol in wheat at 1 ppm
Romer Labs Inc. AgraStrip Total Aflatoxin Test (Product #COKAS1000) - Aflatoxin in corn at 20 ppb using ethanol/water as an extraction solvent
Romer Labs Inc. AgraStrip Total Aflatoxin Test (Product #COKAS1200) - Aflatoxin in corn at 10 ppb using both methanol/water and ethanol/water as extraction solvents

These test kits were evaluated under GIPSA's Rapid Test Performance Evaluation Program.

GIPSA also renewed approval for the Vicam, L.P. AflaTest® aflatoxin test kit, quantitative monoclonal affinity chromatography kit for official testing of aflatoxin in the national grain inspection system.

Official use of the Vicam, L.P. AflaTest® aflatoxin test kit has been approved to determine total aflatoxin levels in corn, condensed distillers solubles, corn bran, corn flour, corn germ meal, corn gluten feed, corn gluten meal, corn meal, corn soy blend, distillers dried grains, distiller dried grains w/ solubles, flaking corn grits, milled rice, popcorn, rough rice, sorghum, soybeans, and wheat.

Romer Labs, Inc. FluoroQuant Afla (FQ Afla) aflatoxin test kit, quantitative fluorometric test kit has been approved for official testing of aflatoxin in the national grain inspection system.

GIPSA has approved the official use of the Romer Labs, Inc. FluoroQuant Afla (FQA) aflatoxin test kit, Product #COKFA1010 to determine total aflatoxin levels in corn, corn meal, milled rice, popcorn, sorghum, soybeans, and wheat.

Diagnostix EZ-Tox DON test kit, quantitative rapid enzyme immunoassay test kit has been approved for official testing of deoxynivalenol (DON) in the national grain inspection system. GIPSA has now approved the official use of the Diagnostix EZ-Tox DON test kit, Product # 600420, to determine levels of DON in wheat, corn, barley, and malted barley.

Neogen Corporation Veratox DON 5/5 quantitative test kit, Product # 8331, has been expanded to include milled rice for official testing of deoxynivalenol (DON) in the national grain inspection system.

Technical information: Lynn Polston, GIPSA Technical Services Division: Lynn.A.Polston@usda.gov.

For information on GIPSA's Rapid Test Performance Evaluation Program and Proficiency Program, visit GIPSA's Biotechnology web page

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