RFE is a compilation of data in several formats that assists with the accurate identification of fish species. It was developed by US FDA\'s RFE Team of scientists at the Seafood Products Research Center (SPRC, Seattle District), and the Center for Food Safety and Applied Nutrition (CFSAN) to help federal, state, and local officials and purchasers of seafood identify species substitution and economic deception in the marketplace.
The RFE includes for each of a number of commercially relevant fish species for sale in the U.S. market high-resolution photographs as their scanned digital images (jpeg format) of whole fish and their marketed product forms (including fillets, steaks, or whole crustaceans) which may be used for visual comparison to a whole fish in question (or its marketed product form). In addition there is chemical taxonomic information consisting of species-characteristic biochemical patterns which may be compared quantitatively to patterns obtained by an appropriate laboratory analysis of the fish species specimen in question. Currently the RFE includes tissue protein patterns determined by Isoelectric Focusing (IEF) electrophoresis and CO1 Mitochondrial DNA sequence patterns determined by DNA Barcoding.